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  • A method for concentration and purification of human coronavirus HCoV-OC43 using CIM QA monolithic columns A_method_for_concentration_and_purification_of_human_coronavirus_HCoV-OC43_using_CIM_QA_monolithic_columns

    Human coronavirus OC43 (HCoV-OC43) is a frequent cause of respiratory tract illness, ranging from common cold to severe disease. The research on coronaviruses and medical application of coronaviral vectors/vaccines requires a quality material of high purity. Unfortunately, virus preparations are highly contaminated with cell debris and purification requires laborious, cost-ineffective procedures.
    Here, we report a simple and efficient method for coronavirus concentration and purification by the example of HCoV-OC43. To achieve this, virus chromatography was performed on CIM QA monolithic columns (BIA Separations), with immobilized positively charged quaternary amines. The quality of the obtained virus stock was assessed with SDS Page electrophoresis, followed by Western blot analysis. Finally, infectivity of recovered virus was evaluated by titration.

  • Native elution in immunoaffinity chromatography enables highly effective virus purification Native_elution_in_immunoaffinity_chromatography_enables_highly_effective_virus_purification

    Downstream processing of viruses in virus vaccine or virus vector production accounts for up to 70% of the overall production costs. Immunoaffinity chromatography is a powerful purification technique due to its high specificity but is disadvantaged by the fact that the elution of the target molecule requires conditions for disrupting interactions between antigen and the antibody and these are often detrimental for both the immobilized proteins and target antigens, especially viruses. Here we describe the mumps virus purification using monolith-based immunoaffinity stationary phase and recently invented native elution of the bound viruses using amino acid solutions under physiological pH.

  • Chromatographic separation of full and empty AAV8 capsids Chromatographic_separation_of_full_and_empty_AAV8_capsids

    Adeno-associated virus (AAV) vectors of various serotypes are considered to have high potential for gene therapy applications. Currently, manufacturing of AAV vectors faces the challenge of co-production of incompletely formed particles lacking a recombinant viral genome. Empty capsids increase the dose of total AAV administered for efficient transduction and are thought to cause unwanted immunological reactions against the virus. Removal of empty capsids during manufacturing, as well as analysis of empty/full AAV particle content is therefore a critical requirement for any AAV production process. This Application Note demonstrates how CIMmultus QA monolithic columns can be used to remove empty AAV capsids from the product chromatographically in a single step.

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