Leaders in monolith chromatography



No, monoliths are not available in aseptic package. Monoliths in general are sanitized using prolonged contact time with 1 M NaOH (not applicable for columns with immobilized proteins).
For disposable purposes all monoliths can be autoclaved. Make sure that either the inlet or the outlet of the column are open. Autoclaving of monolithic columns for aseptic use is also possible. Please consult This email address is being protected from spambots. You need JavaScript enabled to view it. for details.
Basic matrix of all monoliths is poly – methacrylate.
From the chromatogram of pulse test with acetone (or similar tracer molecule) you read retention time, peak width and peak width at a half hight. With that you can calculate:
N = (tR/σ)2 = 16(tR/w) = 5.5(tR/w1/2)
N – number of theoretical plates, tR – retention time, σ - standard deviation, w – peak width, w1/2 – peak width at a half hight
HETP – high equvalent of theoretical plate, L – separation (column) column lenght.
No. cGMP columns are provided with animal free certificates.
Fast and highly efficient separations of large molecules such as proteins, DNA, or viruses, as well as smaller molecules such as peptides. Extremely fast process development, flow unaffected resolution, flow unaffected dynamic binding capacity, high capacity for large biomolecules, low back pressure, versatility, ease of use.
No. We recommend filtration of samples and all the buffers prior to loading on the column.
In order to prolong the column life time we recommend filtration of all samples and buffers. A filter of 0.45 µm pore size is recommended.
Individual column comes on a cart and it measures 660 mm in total height (the column itself only 552 mm). The widest part of the cart is on the bottom and measures 554 mm on the largest end. So maximal footprint of the column is 554 x 554 mm.