CIM® (Convective Interaction Media) monoliths are highly cross linked, porous monolithic polymers with a well-defined channel size distribution. They are supplied readily packed in specially designed housings (CIMmultus™), at a variety of chemistries and scales.
Polymers consist of:
- Poly(glycidyl methacrylate-co-ethylene dimethacrylate) or
- Poly(butyl methacrylate-co-ethylene dimethacrylate)
The polymer composition of CIM® monoliths allows for exceptional mechanical and chemical stability. Incompressibility at extraordinary high flow rates and resistance to harsh chemical conditions during regeneration and sanitization are basic prerequisites of our material. Extensive reusability studies under real life conditions confirm the long-life cycle of CIM® monoliths.
The channel dimensions of CIM monoliths allow for convective mass transfer and combine excellent separation power with flow characteristics, enabling fast separation of large biomolecules and preserving their functionality.
Figure: Transport of biomolecules inside monolithic channels
Biomolecules are conveyed to the active groups located at the surface of the channels by the bulk flow rate of the mobile phase.
The transport between the mobile and the stationary phase is very fast because there is:
- No diffusion
- No dead-end pores
- No void volume
- No stagnant zones
Consequently, the chromatographic performance of CIM® monoliths is flow-rate independent.
Traditional particle based media vs. CIM® Monoliths
The standardized channel size of CIMmultus™ Disposable monoliths lies in the range of 2 µm and allows for unhindered passage of most commonly used large biomolecules.
Certain applications have special demands, and the use of bigger pore sizes might be beneficial. For this purpose, custom made monoliths with pore sizes in the range of 3 to 6 µm are provided on request. Using this approach, the advantages of CIM® monoliths can be fully capitalized even when working with particularly large biomolecules (e.g. Vaccinia virus).