The Ease of Method Screening & Process Optimization

13Nov2023

CIM® monolithic well plates combine the advantages of monolithic chromatography with the convenience of a standard well plate format. They ensure robustness and reliable results, making them ideal for screening multiple chromatographic conditions simultaneously or high-throughput purification of large biomolecules. Each well contains a CIM monolith that enables high flow rates across the plate, with high capacity and yields.

Choosing the Right Tool for Your Screening Task

CIM® monolithic well plates are a scalable tool, available in 96- and 24-well format, with 0.05, 0.2, and 1.0 mL monolith volumes. Designed following ANSI/SLAS microplate standards, and compatible with vacuum manifold, centrifuge, and positive pressure systems.

CIM monolithic well plates can be used for diagnostic or screening purposes. They enable high-throughput process development through fast and efficient evaluation of binding, washing, elution conditions and other chromatographic parameters. The properties of the medium are directly comparable to CIMmultus chromatographic columns, making CIM monolithic well plates a robust tool in early process development stages. These plates are used for fast, highly efficient screening of samples or purification parameters for nucleic acids, viruses, virus like particles, and proteins. They are reusable and perfectly scalable to the existing portfolio.

Discover, how the screening experiments on CIM well plates helped us achieve a DBC of >6 mg/mL and how the results where transfered to the CIMmultus preparative line.

Increasing dynamic binding capacity of Oligo dT using CIM 96 well Oligo dT plates (Poster)

Increasing Dynamic Binding Capacity of Oligo(dT) for mRNA Purification: Experimental Results Using CIM 96-Well Plates (Article)

Screening binding conditions on CIM® Oligo dT 96-well plate to optimize dynamic binding capacity (Application Note)

Featured Product: CIM® Oligo dT18 Monolithic Well Plate

Learn more on the optimization of the rAAV capture step in the downstream process using monoliths. The selected capture step parameters led to high overall vector recovery (51%) and a significant reduction in impurities.

Optimization of rAAV Capture Step Purification Using SO3 Monolith Chromatography (Article)

CIM® SO3 Monolithic 96-Well Plate for Fast and Efficient rAAV Capture Step Screening After Kryptonase-TFF Pretreatment (Poster)

Fast and semi-automatic optimization of capture step for rAAV purification using CIM® SO3 96-well monolithic plate (Application Note)

Fast & Efficient rAAV Downstream Process Optimisation With CIM Monolithic 96-well plates (Webinar)

Featured Product: CIM® SO3 Monolithic Well Plate

Chromatographic applications in diagnostics call for automated systems and high-throughput analyses in order to cope with the large numbers of samples. CIM Monoliths in the well plate follow the increasing needs of diagnostic laboratories. 

CIM® @Tf-0.2 monolithic 96-well plate for immunoaffinity isolation of transferrin from human plasma (Application Note)

Applicability of CIM® Protein G 96-monolithic plate (Pores 2 um) for glycosylation analysis of human IgG isolated from blood (Technical Note)

Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma  (Article)

Benefits

• Simplicity of operation: no shaking and incubation required.  
• Easy transfer of the results to a larger scale: monoliths in well-plates have the same properties as larger preparative monoliths (CIMmultus®), allowing simultaneous screening of  multiple conditions and easy scale-up. 
• Time & money savings: Increased productivity by quick processing time and reduced sample consumption.
• Monolith technology benefits:  Up to 10-times greater capacities compared to other resin-based columns for large biomolecules due to high surface availability of binding sites.  Low shears allow 3-times higher yields for particles and large biomolecules compared to other purification resins. 

Testimonial

“We have been very happy with the CIM screening plates. They have been incredibly useful for buffer composition screening studies that showed very tight correlation to scaled-up conditions. These are wonderful products that we hope Sartorius will continue to prioritize.”  -Bobby Mills, Senior Development Specialist